Anti-Mucin-1 (MUC1 Glycoprotein, CD227), clone 3H2740

Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. Mucin 1 is a breast cancer associated mucin encoded by the Muc-1 gene. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer, pH 6.0 is recommended, Immunohistochemistry: Frozen sections, ELISA: 1:100-1:1000, Optimal working dilutions to be determined by researcher. Immunohistochemistry Positive Control: Breast carcinoma, Recommended Negative Control: I1904-86K: IgG3 Negative Control, Recommended Secondary Antibodies: I1904-65C: IgG, H&L (HRP) Pab Rb x Mo, I1904-65N: IgG (HRP) Pab Rb x Mo, I1903-07E: IgG (HRP) Pab Rb x Mo, I1904-23: IgG, X-Adsorbed (FITC) Pab Gt x Mo, Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.