Anti-Mucin-1 (MUC1 Glycoprotein, CD227), clone 1.B.878

Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 5-10ug/ml for MUC1 capture in conjunction with the same monoclonal enzyme-conjugated versions, or with M4700-07 specific to carbohydrate portions of MUC1 (detection). , Immunohistochemistry: Frozen sections or neutral-buffered formalin-fixed paraffin-embedded sections., Optimal dilutions to be determined by the researcher. Matched Pairs: Capture: M4700-08, M4700-06D , Detection: M4700-07, M4700-08 , Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice. Affinity Constant: >1x10e8, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.