Anti-MIF (Migration Inhibitory Factor), Biotin conjugated

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of macrophages. Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of imflammation and immunity. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. HL-60 human acute promyelocytic leukemia cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin., Western Blot: 0.1ug/ml. Recombinant human MIF., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS Aliquot to avoid repeated freezing and thawing. Store at -20°C. . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.