Anti-Microtubule-associated Protein 2 (MAP2, MAP-2)

MAP2 is a type II microtubule associated protein (MAP). It is located predominately in dendrites where it accelerates tubulin polymerization, cross-links microtubule fibers into stable bundles, and connects microtubule filaments to intermediate filaments. Alternative splicing of MAP2 mRNA gives rise to at least three different isoforms including, MAP2a (280kD), MAP2b (200kD), and MAP2c (70kD). The MAP2c isoform is expressed in embryos, whereas the MAP2a and MAP2b isoforms are expressed in adult tissues. MAP2 is the somatodentritic MAP of neurons, but it also is expressed to a lesser extent in glia. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml, Immunohistochemistry: Frozen, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.