Anti-MEK1, NT (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1)

MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Phosphorylation by Mek1 increases Erksí ability to phosphorylate micrtubule-associated proteins and myelin basic proteins. Mek1 is involved in diverse arrays of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation (3), and DNA recombination during meiosis. Mek1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway. , , Applications: , Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Colorimetric): 1ug/ml, Immunohistochemistry: 1:50, Optimal dilutions to be determined by the researcher. Positive Controls: H1838-01: HeLa Cell Lysate, Heat Shocked, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.