Anti-MEK1, MEK2 (Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MA

MEK1 and MEK2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK1/2 is a critical step in the MAP kinase cascade. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.