Anti-MEK1 (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1)

MEK1 is one of the MEKs that are upstream of the MAPK ERK1/2. MEK1 is a dual-specific protein kinase that phosphorylates ERK1/2 on both threonine and tyrosine residues in the sequence TEY. Phosphorylation of these residues dramatically activates ERK1/2, which then phosphorylate their downstream substrate. MEK1 contains 393 amino acids and has a calculated molecular weight of 43,470. MEK1 itself is regulated by phosphorylation by one of the MEK kinases. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunoprecipitation: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.