Anti-MEK1 (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1), clone 3G188

MEK (MAP kinase or ERK kinase) is also known as MAP kinase kinase (MAPKK). MEK1 is a dual tyrosine/threonine kinase that phosphorylates and activates MAP kinase (ERK1 and ERK2). MEK1 has a molecular weight of 43.5kD and contains 393 amino acids. MEK1 is phosphorylated and activated by serine/threonine kinases via 2 different pathways(3). These kinases include, c-raf-1, v-raf-1, c-Mos, and MEKK. MEKK shares homology with yeast kinases Ste11 and byr2. MEK2 has been identified and cloned(4). MEK2 differs from MEK1 in the amino terminal region and between the conserved kinase subdomains 9 and 10. The MEK1 murine gene is found on chromosome 9 the MEK2 gene on chromosome 10. It is likely that more members of the MEK family exist. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 , Immunoprecipitation: 2-5ug , Optimal dilutions to be determined by the researcher. Positive Control: Mouse brain cell lysates, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.