Anti-MCM2 (Minichromosome Maintenance Protein 2, BM28), clone 3H2645

MCM2 (Mini chromosome maintenance protein-2), is a protein involved in the control of DNA replication. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen): 1:500-1:1000, , Immunohistochemistry (paraffin): 1:200, Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections (0.01M cutrate buffer, pH 6.0)., Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo, I1904-66C: IgG, F(ab')2, X-Adsorbed (HRP) Pab RbxMo, Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control, Hybridoma: Sp2 myeloma cells with spleen cells from CBA/c F1 mice. Recommended Positive Control Tissue: Tonsil, any tissue with high proliferative index, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.