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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
M2749-01E.20 | 20 µl | - | - |
3 - 19 business days* |
395.00€
|
||
M2749-01E.100 | 100 µl | - | - |
3 - 19 business days* |
696.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human... more
Product information "Anti-Mcl 1 (Myeloid Cell Leukemia 1, MCL1, Mcl-1, Bcl 2 Related Protein EAT/Mcl1, Bcl-2-like Protein"
Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes proapoptotic Bcl-2 family members (3), and inhibits apoptosis induced by a number of cytotoxic stimuli (4). Mcl-1 differs from its other family members in its regulation at both the transcriptional and post-translational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life (1,2). Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation (1,5-7). Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal (8-11). Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover (10) but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization (11). Mcl-1 deficiency in mice results in peri-implantation lethality (12). In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes (13). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-mcl1/EAT, Anti-Bcl-2-like protein 3, Anti-Bcl-2-related protein EAT/mcl1, Anti-Induced myeloid leukemia cell differentiation protein Mcl-1 |
Supplier: | United States Biological |
Supplier-Nr: | M2749-01E |
Properties
Application: | WB |
Antibody Type: | Monoclonal |
Clone: | 11C1047(D35A5) |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey, mouse |
Immunogen: | Synthetic peptide corresponding to residues surrounding Leu210 of human Mcl-1. |
Format: | Purified |
Database Information
KEGG ID : | K02539 | Matching products |
UniProt ID : | Q07820 | Matching products |
Gene ID | GeneID 4170 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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