Anti-MCAF (MBD1-containing Chromatin Associated Factor, MCAF1, Activating Transcription Factor 7 Int

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:20,000, Immunohistochemistry (Frozen/paraffin): 1:10-1:500, Optimal dilutions to be determined by the researcher. Positive Control: HeLa Nuclear Extract, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.