Anti-MAP Kinase, p38-2 (MAPK, Erk2, Extracellular Signal Regulated Kinase, RK, CSBP, Mitogen Acti

p38 (CSBP, RK, or Mpk2) is among the newest MAPK family members to be isolated. This protein was originally identified as a kinase which was phosphorylated on tyrosine in response to treatment of cells with LPS. Subsequently, p38 (CSBP1/CSBP2) was identified as the target of pyridinyl-imidazole compounds (eg: SB 203580) which inhibit the production of interleukin-1 and tumor necrosis factor from stimulated human monocytes. CSBP1 and CSBP2 are splice variants of p38 differing only in an internal 25 amino acid sequence. p38 and JNKs appear to be activated by many of the same stimuli, nevertheless, differences in the extent and timing of activation of p38 and JNK have been observed. Further, p38 and JNKS are activated by distinct MAPK kinases. For example, MKK4 activates both JNK and p38, whereas MKK3 and MKK6 activate p38 specifically. Studies have implicated p38 in the phosphorylation of the small heat shock proteins Hsp27, MAPKAP kinase-2 and-3, and the transcription factors ATF2, Elk-1 and CHOP. In addition, p38 likely mediates increased cytokine expression and some aspects of programmed cell death. Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.1 - 1.0ug/ml, Western Blotting: 1-3ug/ml , Immunoprecipitation: 5-10ug/IP reaction , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.