Anti-Macrophage, Low Endotoxin (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200, Membrane permeabilization is required., Immunohistochemistry (Frozen/Paraffin): 1:100-1:200, Paraffin sections require protein digestion pre-treatment (e.g. 0.1% trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment., Optimal dilutions to be determined by the researcher. Recommended Positive Control: Human Spleen, Hybridoma: NS1 myeloma cells with spleen cells from BALB/c mice. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-14C: IgG, Fc (HRP) Pab Gt xMo, I1904-19C: IgG, F(ab')2 (HRP) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.