Anti-LRP4 (Low Density Lipoprotein Receptor-related Protein 4, KIAA0816, LRP10, LRP-4, MEGF7, Multip

LRP-4 (Low density lipoprotein-related protein 4, also MEGF7 and LRP13) is a 215-225kD member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP-4 is an 1885aa type I transmembrane glycoprotein. It contains a 1705aa extracellular domain (ECD) (aa21-1725) plus a 159aa cytoplasmic region (aa1747-1905). In the ECD, there are eight LDLR class A repeats (aa26-350), two EGF-like repeats (aa354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa480-1610). Over aa18-344, human LRP-4 shares 88% aa identity with mouse LRP-4., , Applications:, Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human LRP-4 is ~0.5ng/ml., Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.