Anti-Leptin (Ob, Obese Protein)

Applications: , Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 (ECL). The obese protein is predicted to be secretory protein with a molecular weight of ~ 16kD, ELISA: 1:10,000-50,000 using 10-100ng of Leptin protein/well). Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.