Anti-LAMP3 (Lysosome-associated Membrane Glycoprotein 3, LAMP-3, Lysosomal-associated Membrane Prote

MaxLightT490 is a new Blue-Green photostable dye conjugate comparable to DyLightT488, Alexa FluorT488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms. , Applications:, Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (FFPE): Requires HIER using sodium citrate buffer pH6.0, Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.