Anti-Laminin B2, gamma1, clone 0.N.384 (A5)

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G., Immunohistochemistry (Acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 minutes at RT. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37°C., Optimal dilutions to be determined by the researcher. Positive Control: LS174T cells, normal colon or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.