Anti-Lamin A

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1,3). Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation. During apoptosis, the 70 kD lamin A is specifically cleaved to a large (40-45 kD) and a small (28 kD) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot 1:1000 , Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:200 , Flow Cytometry: 1:50 , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.