Anti-LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223), Biotin conjugated, clone 6D65

The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36kD) and LAG-3V3 (D1-D3, 52kD). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-gamma production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. Applications: Suitable for use in ELISA, Western Blot. Other aapplications not tested. Recommended Dilutions: ELISA: Detection., Western Blot: 5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.