Anti-IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)

IRF1(interferon regulatory factor1) is a 45kD member of the IRF family of proteins. It is induced by TLR9 and INF stimulation, and positively regulates theexpression of IFNb NOS and1L12p35., Human IRF1 is 325aa in length. It contains an Nterminal DNA binding basic region (aa1140), and an acidic Cterminus(aa147-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11aa substitution for the C terminal 86aa. Over aa171-325,human IRF1 shares 83% aa identity with both canine and porcine IRF1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.