Anti-Interleukin 9 Receptor (IL-9R, CD129)

Interleukin 9 Receptor (IL-9 R) belongs to the hematopoietin receptor superfamily and is the binding subunit of the heterodimeric IL-9 receptor complex. The other subunit is the common gamma chain shared with the receptors for IL-2, IL-4, IL-7, and IL-15. IL-9 R is expressed by T cells, neutrophils, mast cells, and macrophages. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Sf 21-derived, recombinant human IL-9 R (rhIL-9 R, aa 40-270, Accession # NP_002177) extracellular domain. Endotoxin Level: < 0.1 EU per 1ug of the antibody as determined by the LAL method. Applications: Neutralization of bioactivity, Flow Cytometry, Direct ELISA , Recommended Dilution: Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4ug/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10e5 cells/mL) in the presence of 2 ng/mL of rhIL-9., Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50ug/mL and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. , Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.