Anti-Interleukin 9 (IL-9)

Applications: , Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells. , Direct ELISA: 0.5-1.0ug/ml detection limit for rrIL-9 is ~ 1ng/well., Western Blot: 0.1-0.2ug/ml detection limit for rrIL-9 is ~ 5ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.