Anti-Interleukin-8 (IL-8)

IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5ug/ml, Western Blot: 0.1-0.2ug/ml, Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Sheep anti-rabbit IgG labeled with HRP, Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.