Anti-Interleukin 8 (IL-8)

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72-amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml , Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.