Anti-Interleukin 8 (IL-8)

CXCL8, also known as IL-8, is a chemoattractant that recruits neutrophils to sites of inflammation, activates neutrophil functions and promotes angiogenesis. CXCL8 production is rapidly induced by pro-inflammatory signals in many different cell types. The biological activites of CXCL8 are mediated by chemokine receptors CXCR1 and CXCR2. Feline CXCL8 is translated as a 101 aa precursor protein with a 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine CXCL8, respectively. Applications: Suitable for use in Western Blot, Neutralization and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, ELISA: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.