Anti-Interleukin-8 (IL-8), clone 3H209

IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilution: Neutralizing: In chemotaxis assay, the antibody inhibited 97.8% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/ml of I8430-02E and 10ng/ml of human IL-8 in assay. I8430-02E inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody's ability to neutralize IL-8 activity. I8430-02E has shown neutralizing activity on calcium ion changes in human granulocytes using FACS. , Western Blot: 0.02-0.1ug/ml allows visualization of 100ng/lane of human IL-8., Optimal dilutions to be determined by researcher. Matched Pair: I8430-02A: Dectection, I8430-02E: Capture, Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.