Anti-Interleukin-8 (IL-8), clone 3H205

IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA, Neutralizing and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.3ug/well Substrate: TMB. , Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/ml of I8430-02A and 10ng/ml of human IL-8 in assay. I8430-02A inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody's ability to neutralize IL-8 activity. I8430-02A has shown neutralizing activity on calcium ion changes in human granulocytes using FACS. , Western Blot: 0.02-0.1ug/ml allows visualization of 100ng/lane of human IL-8., Optimal dilutions to be determined by researcher. Recommended Matched Pair: I8430-02A (Dectection): Interleukin 8 (IL-8), I8430-02E (Capture): Interleukin 8 (IL-8), Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.