Anti-Interleukin 7 Receptor alpha (IL7Ra, CD127, CDw127, L7RA, IL-7R-alpha, ILRA)

IL-7 R-alpha (IL-7 receptor alpha, also CD127) is a 60-70kD member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R-alpha heterodimerizes with both the gc chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R-alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R-alpha is a 437aa type I transmembrane glycoprotein. It contains a 219aa extracellular region (aa21-239) that possesses one type III fibronectin domain (aa129-222), and a 193aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.