Anti-Interleukin 6 (IL-6)

Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Direct ELISA: >0.5ug/ml using 100ul/well antibody solution. Allows the detection of 0.2-0.4ng/well of recombinant human IL-6., Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human IL-6 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions., Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-6 (1ng/ml), a concentration of 0.2-0.4ng/ml is required., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.