Anti-Interleukin-6 (IL-6), clone 5J2

Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in Flow Cytometry, Western Blot and Dot Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.25ug stains 10e6 rat peritoneal macrophages stimulated with LPS., Western Blot: 0.1-1ug/ml, Dot Blot: 0.1-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.