Anti-Interleukin-6 (IL-6), clone 3H2541

Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications have not been tested. , Recommended Dilutions: ELISA: 5ug/ml (as a coating antibody), Flow Cytomtery: Use 10ul to label 1x10e6 cells in 100ul. Membrane permeabilization is required., Optimal dilutions to be determined by the researcher. Matched Pair: Capture: I8428-22X, Detection: I8428-03J, Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.