Anti-Interleukin-5 (IL-5), clone 5K121

Applications: , Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence/Flow Cytometry: For intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations., Western Blot: 1-5ug/ml (<100ng/lane) Detects recombinant human IL-5 under reducing conditions. , Optimal dilutions to be determined by the researcher. Matched Pair: Capture: , I8427-06J Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF), Dectection:, I8427-06A Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin), Recommended Secondary: Western Blot:, I1904-47J IgG, H&L (AP) GtxRt, I1904-47P IgG, H&L, X-Adsorbed (AP) GtxRt, I1904-47U IgG, H&L (AP) GtxRt, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.