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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
I8426-23X.500 | 500 µg | - | - |
3 - 19 business days* |
539.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses... more
Product information "Anti-Interleukin-4 (IL-4) (azide-free), clone 3H2534"
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Neutralization. Other applications not tested. Recommended Dilution: ELISA: Useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels. Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard., Western Blot: 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <100ng/lane of recombinant human IL-4 under reducing conditions., Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control., Neutralization: Can be used for neutralizing human IL-4 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | I8426-23X |
Properties
Application: | ELISA, FC, IF, FA (Neutr.), WB |
Antibody Type: | Monoclonal |
Clone: | 5K302 |
Conjugate: | No |
Host: | Rat |
Species reactivity: | human |
Immunogen: | CHO-expressed recombinant human IL-4 |
Format: | Purified |
Database Information
Handling & Safety
Storage: | -20°C (frozen cool packs!) |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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