Anti-Interleukin 33 (IL33, Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear F

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a member of IL-1 cytokine family. IL-33 is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 Alpha or Beta in arterial smooth muscle, dermal fibroblasts, and keratinocytes. IL-33 is synthesized as a 270aa, 32kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature human IL-33 shares 55% aa identity with mouse IL-33. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.