Anti-Interleukin-32 (IL-32, Natural Killer Cells Protein 4, NK4)

Description:, Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2 (1). It was later re-isolated from an IL- 18-treated lung carcinoma cell line and re-named IL-32 , (2). IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases , such as rheumatoid arthritis (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2.5-5ug'ml, Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,