Anti-Interleukin-3 (IL-3), clone 5K130

Applications:, ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels. Purified antibody should be paired with biotinylated antibody (Cat. No. I7663-38V) as the detection antibody, with purified recombinant mouse IL-3 as the standard., Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-3-positive cells within mixed cell populations. Recombinant mouse IL-3, unlabeled (Cat. No. I7663-39T), or Rat IgG1 should be used as a specificity control. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.