Anti-Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE)

IL-23R is an IL-23-specific binding protein that associates with IL-12 Rb1 to form a functional heterodimeric receptor complex. This complex mediates biological activities that are similar to, but distinct from, those induced by IL-12. IL-23R is expressed by Th1 and NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. 10ul labels 1x10e6 cells., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction., 2. Transfer 25ul of the Fc-blocked cells (1x10e6 cells) or 50ul of packed whole blood to a 5ml tube. , 3. Add 10ul of I8443-49A. , 4. Incubate for 30-45 minutes at 4°C. , 5. Following this incubation, remove unreacted I8443-49A reagent by washing the cells twice in 4ml of the same PBS buffer (Note: Whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). , 6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis. , 7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.