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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
I8443-18F.500 | 500 µg | - | - |
3 - 19 business days* |
1,189.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Interleukin 22 (IL-22), also called IL-10-related T cell-derived inducible factor or IL-TIF, is a... more
Product information "Anti-Interleukin 22 (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)"
Interleukin 22 (IL-22), also called IL-10-related T cell-derived inducible factor or IL-TIF, is a secreted cytokine that contributes to the inflammatory response in vivo. IL-22 is expressed in T cells and mast cells in response to IL-9 or lipopolysaccharide. IL-22 monomers bind a class II cytokine receptor that is a heterodimer of CRF2-9 (IL-22 Ra1) and CRF2-4 (IL-10 Rb). Mouse IL-22 is a 179 aa protein with a 33 aa signal peptide, three N-glycosylation sites and two intramolecular disulfide bonds. The mature protein shows 78% and 90% aa identity with human and rat IL-22, respectively. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml. Detection limit for recombinant mouse IL-22 is ~1ng/lane under non-reducing and reducing conditions. , Flow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-5 x10e5 cells in a total reaction mixture not exceeding 200ul. For intracellular staining with PMA/ionomycin- stimulated EL-4 cells to detect IL-22, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Secondary antibody such as anti-rat IgG conjugated to a fluorochrome can be used. , Direct ELISA: 0.5-1ug/ml. Detection limit for recombinant mouse IL-22 is ~2.5ng/well. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-Il22, Anti-IL-22, Anti-Il22a, Anti-IL-22a, Anti-IL-TIF, Anti-IL-TIF alpha, Anti-Interleukin-22, Anti-Interleukin-22a, Anti-IL-10-related T-cell-derived-inducible factor |
Supplier: | United States Biological |
Supplier-Nr: | I8443-18F |
Properties
Application: | ELISA, FC, WB |
Antibody Type: | Monoclonal |
Clone: | 8H107 |
Conjugate: | No |
Host: | Rat |
Species reactivity: | mouse |
Immunogen: | E. coli-derived rmIL-22 |
Format: | Affinity Purified |
Database Information
KEGG ID : | K05445 | Matching products |
UniProt ID : | Q9JJY9 | Matching products |
Gene ID | GeneID 50929 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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