Anti-Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha, CD25, IDDM10, Ly43, p55, T Cell Growth Fact

The CD25 cell surface antigen is a 55kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells. Blocks the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:5, 10ul labels 10e6 cells in 100ul, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.