Anti-Interleukin-2 (IL-2) FITC conjugated, clone 6A421

IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation. Interleukin-2, clone 3H2514 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it acts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkins et al. 1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotze et al. 1986). This antibody is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talal et al. 1983). , Applications:, Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required., Optimal dilution determined by the researcher, Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.