Anti-Interleukin-2 (IL-2), clone 4H135

Applications:, Sutable for use in ELISA, Immunoprecipitation and Western Blot. Other applicastions not tesed. Recommended Dilution: ELISA: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-2 protein levels. Purified antibody may be paired with biotinylated antibody (Cat. No. I7663-27M5) as the detection antibody, with purified recombinant mouse IL-2 as the standard. Or, conversely, biotinylated antibody may be paired with purified JES-5H4 antibody. Western Blot: A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of < 100ng/lane of recombinant mouse IL-2 under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.