Anti-Interleukin-16 (IL-16)

Interleukin-16, also known as lymphocyte chemoattractant factor (LCF), is a chemotactic cytokine that stimulates a migratory response in CD4+ lymphocytes, monocytes and eosinophils through binding to the CD4 receptor. IL-16 induces human T-lymphocyte expression of interleukin 2 receptor and upregurates major histocompatibility complex molecules on resting human T lymphocyte. It is able to render CD4+ T cells unresponsive to TCR/CD3 stimuli. Moreover, IL-16 inhibits the replication of T cell and monocyte tropic HIV strains in vitro. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tseted. Recommended Dilution: Neutralization: 0.12-0.15ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2ng/ml)., ELISA: >0.5ug/ml using 100ul/well antibody solution. Allows the detection of 0.2-0.4ng/well of recombinant hIL-16., Western Blot: 0.1-0.2ug/ml The dectection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.