Anti-Interleukin 15 Receptor alpha (IL-15R alpha, IL-15Ra)

Applications: , Suitable for use in Neutralization of Bioactivity, Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Neutralization of Mouse IL-15Ra Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15Ra mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15Ra mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. The ND50 for this lot of anti-mouse IL-15Ra antibody was determined to be ~0.3-1.5ug/ml in the presence of 0.8ng/ml of human IL-15, using the CTLL-2 cell proliferation assay. , Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse IL-15Ra. The detection limit for rmIL-15Ra is ~0.1ng/well. In this format, this antibody shows ~5% crossreactivity with rhIL-15Ra and less than 1% crossreactivity with rhIL-2Ra, rmIL-2Rb and rmIL-2Rg. , Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse IL-15Ra. The detection limit for rmIL-15Ra is ~5ng/lane under non-reducing and reducing conditions. , Flow Cytometry: 3-10ug/ml/10e6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.