Anti-Interleukin-13 (IL-13) Biotin conjugated, clone 5K127

Applications:, ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1,2 Biotinylated antibody should be paired with purified antibody (Cat. No. I8435-12G) or (Cat. No. I8435-05A) as the capture antibody, with purified recombinant human IL-13 as the standard., Western Blot: Useful for Western blot applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of < 100ng/lane of recombinant human IL-13 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched ELISA pairs: Capture: I8435-12G or I8435-05A , Dectection: I8435-13A, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.