Anti-Interleukin-13 (IL-13) (azide-free), clone 1.B2.1

Applications:, ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels. Purified antibody should be paired with biotinylated antibody (Cat. No. I8435-13A) as the detection antibody, with purified recombinant human IL-13 as the standard., Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control., Western Blot: Useful for Western blot applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of < 100ng/lane of recombinant human IL-13 under reducing conditions., Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.