Anti-Interleukin-12 (IL-12), clone 3H2556

Applications:, Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen, ELISA: 1:1000 (detection antibody), Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Rabbit anti-mouse IgG labeled with horseradish peroxidase (HRP):, I1904-65W: IgG, F(ab')2 (HRP) Pab Rb xMo, I1904-66C: IgG, F(ab')2, X-Adsorbed (HRP) Pab Rb xMo, Recommended Negative Controls: I1904-78M: IgG1 Negative Control, Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2-O myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.