Anti-Interleukin 10R, beta (IL-10 R-beta, Interleukin 10 Receptor beta, IL-10R2, CD210b, CRF2-4) (AP

The IL-10 receptor consists of two chains, a ligand binding IL-10 Ra (IL-10 R1) chain and a signaling chain known as IL-10 Rb (IL-10 R2). The human IL-10 Rb chain is a 325aa protein with 69% homology to its mouse counterpart. Stimulation of cells by IL-10 activates both Jak1 and Tyk2 kinases. The IL-10 Rb chain is also a component of the recently identified IL-22 receptor. Both chains of the IL-10 R are expressed on lymphocytes, monocytes, and neutrophils, with the IL-10 Rb chain expressed at higher levels than the IL-10 Ra chain in most cases. IL-10 signaling can affect a variety of immune functions, including inhibition of pro-inflammatory cytokine synthesis and regulation of the growth and function of B cells and antigen-presenting cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul to label 1x10e6 human peripheral blood monocytes, Optimal dilutions to be determined by the researcher. Hybridoma: myeloma cells with B cells Balb/c mice. Absorption: 620-650nm , Emission: 660-670nm, Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. For maximum recovery of product, centrifuge the original vial prior to removing the cap.