Anti-Interleukin 10 Receptor alpha (IL10RA,, IL-10RA, IL-10R-A, CDw210a, HIL-10R, IL-10R1, IL10R)

Interleukin-10 (IL-10) is a cytokine whose main biological function is to suppress the immune response by induction of a signal(s) leading to inhibition of synthesis of a number of cytokines and their cellular receptors. Signal transduction is initiated upon formation of a ternary complex of IL-10 with two of its receptor chains, IL-10R1 and IL-10R2, expressed on the cell membrane. The affinity of IL-10R1 toward IL-10 is very high, which allowed determination of the crystal structure of IL-10 complexed with the extracellular/soluble domain of IL-10R1, while the affinity of IL-10R2 toward either IL-10 or IL-10/sIL-10R1 complex is quite low. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50, 10ul labels 10e6 cells in 100ul, Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.