Anti-Interleukin-10 (IL-10)

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. Applications: Suitable for use in Neutralization, Sandwich ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Sandwich ELISA: 0.5-2ug/ml to detect rat IL-10 using 100ul/well antibody solution. Used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant rat IL-10., Western Blot: 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , , Country of Origin: USA