Anti-Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004). Applications: Suitable for use in Flow Cytometry, ELISA and Functional Assays. Other applications not tested. Recommended Dilution: ELISA: 5-10ug/ml, Flow Cytometry: membrane permeabilization is required for this application, Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.